eDNA Frequently Asked Questions
General Information
Q: What is eDNA and how is it used to detect parasites in fisheries?
A: Environmental DNA (eDNA) is genetic material shed by organisms into their environment. It can be collected from water, soil, or sediment samples and analysed to detect the presence of specific organisms, including parasites.
Q: Why is it important to detect parasites in coarse fisheries?
A: Detecting parasites is crucial for maintaining fish health, ensuring sustainable fisheries management, preventing the spread of diseases, and protecting both wild and farmed fish populations.
Sample Collection and Submission
Q: What types of samples are needed for eDNA testing of parasites from environment?
A: Water samples are typically collected for eDNA analysis, but sediment, biofilm or non- lethal samples including skin or gill mucus from fish species can also be used depending on the target parasite and environmental conditions.
Q: How should I collect water samples for eDNA analysis?
A: We recommend using our “Mainstream water eDNA sampling kit”. Detailed sampling instructions are provided in our Sample Collection Guide.
Q: How do I submit samples to your laboratory?
A: After collecting the samples, follow the packaging and shipping instructions provided in our sample collection guide section. Ensure samples are kept cool and shipped to our laboratory as quickly as possible. You need to take the sample and post it back to us within 48 hours.
Q: What is the recommended volume of water for eDNA testing?
A: This depends on the size of the water body and disease factors – see choice of eDNA kits.
Testing and Results
Q: How long does it take to receive results from eDNA testing?
A: Results are typically available within 5-7 working days after the samples are received at our laboratory. More rapid services may be available upon request.
Q: What parasites can be detected using eDNA in coarse fisheries?
A: Our eDNA assays can detect a range of common fish parasites. For full list of available eDNA assays, please see Parasite Detection in Coarse Fisheries
Q: How accurate and reliable are the eDNA results?
A: eDNA is highly sensitive and specific, capable of detecting low levels of parasite DNA in the environment. However, results can be influenced by factors such as sample quality, sample volume, environmental conditions, nature of target host and abundance of shedded eDNA in the surrounding environment, and the presence of inhibitors.
Applications and Benefits
Q: How can eDNA testing benefit coarse fisheries?
A: eDNA qPCR testing provides early detection of pathogens and invasive aquatic species, allowing for timely intervention and management strategies to prevent outbreaks and minimize impacts on fish populations and fisheries.
Q: Can eDNA testing help in managing disease outbreaks?
A: Yes, by providing rapid and accurate detection of fish parasites, eDNA testing helps fisheries managers monitor parasite levels and implement control measures to manage and prevent outbreaks effectively.
Q: What should I do if I have questions or issues with my eDNA results?
A: If you have any questions or encounter issues with your results, please contact our customer service team. Telephone 01790 753324 or email info@mainstreamfisheries.co.uk
We are dedicated to resolving any concerns and ensuring your satisfaction.